Measurement of Human Endothelial Cell Adhesion Using Dielectrophoresis
摘要
This study measures adhesion force of human endothelial cell (ECV304) using dielectrophoresis. This study cultures ECV304 cells on polydimethylsiloxane (PDMS) surface and glass surfaces. Experimental results show that the ECV304 cells can attach and reproduce on the PDMS surface and the glass surface with a collagen coating (Rat Tail Tendon Type I) at 37℃. At a seeding time of 1 hour, the cell adhesion force on the glass surface and on the PDMS surfaces is about 32% and 21% of the cell adhesion force at a seeding time of 7 hours, respectively. The cell adhesion force increases as the seeding time increases, for both PDMS and glass surfaces. The cell adhesion strength increases at the early stages of adhesion and after the seeding time of 6 hours because of the focal adhesion kinase activation. In addition, cell seeded on the glass exhibited better cell adhesion than cells seeded on PDMS, perhaps due to partial denaturation or changes in integrin orientation on the hydrophobic surface.