[A study on the loss of heterozygosity of multiple tumor suppressor genes in gastrointestinal metaplasia].
摘要
OBJECTIVE: To investigate the role of multiple tumor suppressor gene abnormality in gastrointestinal metaplasia (IM). METHODS: The loss of heterozygosity (LOH) of YNZ22, APC, MCC and DCC genes was examined by PCR-RFLP technique in 60 IM cases. RESULTS: LOH of YNZ22, APC, MCC and DCC genes were found in 19.4% (6/31), 12.0% (3/25), 13.0% (3/23) and 4.3% (2/47) respectively. IM was classified into types I, II and III. It was found that the LOH rates of each tumor suppressor gene of type III IM were higher than those of types I & II IM, but the difference was not significant (P > 0.05). Two cases of type III IM were found to also have 2 gene alterations (APC, MCC or YNZ22, MCC) respectively. All LOH were found in IM of adjacent carcinoma. None were found in IM of cancer-free patients. CONCLUSION: The alteration of tumor suppressor gene may play a role in the development and carcinogenesis of IM.