[Studies on the committed differentiation of CD34+ hematopoietic progenitor cells].
摘要
OBJECTIVE: To elucidate the capacity of different hematopoietic growth factors (HGFs) combinations for inducing CD34+ cells to proliferate and differentiate committedly to granulocytes, erythrocytes, megakaryocytes and dendritic cells (DC). METHODS: CD34+ cells were isolated from umbilical cord blood by using a high-gradient magnetic cell sorting system (MACS), and expanded with HGFs in a liquid culture system. Colony forming cells and antigen expression were studied by colony forming assays and FACS. RESULTS: Different combinations of HGFs, including SCF, IL-3, IL-6, GM-CSF, Epo and Tpo increased BFU-E, CFU-GM, CFU-MK and CD41a+ cells by 14.97 +/- 2.89 fold, 14.46 +/- 3.19 fold, 34.67 +/- 4.62 fold and 17.29 +/- 2.34 fold, respectively. Combination of HGFs allowed generation of a large number of DCs. Cultures of CD34+ cells with the combination of FLT-3 ligand + GM-CSF + IL-4 + TNF-alpha yielded 24.28% +/- 2.14% CD1a+ cells, while the control cultures did not. CONCLUSION: It is possible to induce CD34+ cells to proliferate and differentiate committedly to different lineage of hematopoietic cells and DC.