[Development of a system for Polymyxa graminis infection and transmission of wheat yellow mosaic virus].
摘要
In growth chambers, a system was developed for infection of Polymyxa graminis to wheat(Triticum aestivum L.), which is an obligate parasite. By the system, the fungus could be propagated in large quantity rapidly and the life cycle of P. graminis in wheat was observed in a period of 13 to 15 days. The methods for separating P. graminis as single cystosori from cultured wheat roots or dried roots were simplified. By tests for the factors affecting on P. graminis infection, including inocula types, seedling age of host plants, temperatures, pH value and nutrient contents etc., the system was modified to more perfection. With an integration of optimal temperature and other elements in the system, which affect on infection and development of P. graminis, wheat yellow mosaic virus (WYMV) was transmitted constantly and efficiently by the fungal vector. In average, 70% of wheat plants inoculated by the viruliferous fungi could infected by WYMV and the typical symptom of wheat mosaic disease would appear at 30 days post-inoculation mechanically.