[Effects of hepatitis B virus X gene on apoptosis and the activity of telomerase in HepG(2) cells].
摘要
OBJECTIVE: To observe the effects of hepatitis B virus X gene on hepatic carcinoma. METHODS: The expression vectors of HBs gene and HBc gene (pCEP4-S and pCEP4-C) were transfected into HepG(2) cells by electroporation technique, and the cells, HepG(2) cells which contained the expression vectors of HBs (pCEP4-X) were recovered and cultured stably. The activity index of telomerase and the apoptosis rates in three cell lines were determined by PCR-ELISA and flow cytometry. RESULTS: The 39.50% of cells expressed HBx gene came into S-phase of cell cycle, and the activity index of telomerase was higher in the cell line (3.95 +/- 0.07) than in other cell lines and control (F=20.85, P<0.01; d'=2.15, P<0.05) after synchronized culture. Induced by antisense oligonucleotides against telomerase the apoptosis of cell line expressed HBx gene reduced (apoptosis rate: 1.75%). The activity of cells also reduced with the dose increase of the antisense oligonucleotides and it showed a dose-response relationship. CONCLUSION: The activity of HepG(2) cell can be increased and the cell apoptosis can be reduced by HBx gene. It may be another malignant process of hepatocytes.