[Relationship of XRCC1 polymorphism with the risks and clinicopathological factors of cervical cancer].
摘要
OBJECTIVE: To explore the correlation of XRCC1 Arg194Trp Arg399Gln SNPs with the risks and clinicopathological factors of cervical cancer. METHODS: Polymorphisms Arg194Trp, Arg399Gln of XRCC1 gene in 253 cervical cancer patients and 350 healthy controls were analyzed by mismatch amplification polymerase chain reaction (MAMA-PCR). RESULTS: Compared with the Arg/Arg genotypes, the Trp/Trp genotypes could increase the risk of cervical cancer (P = 0.000) . Arg/Arg was a protection factor with an odds ratio of 0.116 (95%CI = 0.043-0.316) . The frequency of Arg and Gln allele among cervical cancer and healthy controls were 56.81%, 43.19% and 64.86%, 35.14%. Compared with the Arg genotypes, the Gln genotypes could increase the risk of cervical cancer (P < 0.05) . Compared with the Arg/Arg genotypes, the Gln/Gln genotypes could increase the risk of cervical cancer (P < 0.01) . Arg/Arg was a protection factor with an odds ratio of 0.188 (95%CI = 0.089-0.398) . Using haplotypes 194Trp-399Gln as a reference, women carrying 194Arg-399Gln and 194Arg-399Arg halpotypes had a significantly decreased risk for cervical carcinoma. The adjusted OR and 95%CI were 0.613 [0.446, 0.842] and 0.635 [0.449, 0.899]. The frequencies of Arg/Arg, Arg/Gln, Gln/Gln genotype had significant relationship with clinical stage and histological grade. Tumor diameter, patient age and neoplastic form had no relationship. CONCLUSION: Compared with the Arg/Arg genotypes, the Trp/Trp and Gln/ Gln genotypes could increase the risk of cervical cancer. The Trp or Gln genotypes risk increases. Arg may be a protection factor. The frequencies of Arg399Gln SNP have a correlation with stage and histological grade.