[Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors].
摘要
The exogenous gene cyclinD1 cDNA was inserted into eukaryotic expression vector pXJ41-neo by gene transduction and recombination techniques. The recombinant was detected by electrophoresis. Results showed that the recombinant sense plasmid (pXJ41-cyclinD1) was digested by HindIII into 229 bp, 427 bp and 7554 bp; while the recombinant antisense plasmid was digested into 427 bp, 1184 bp and 6599 bp, implying that sense and antisense pXJ41-cyclinD1 eukaryotic expression vectors were successfully recombined. The finding from this study offers an effective tool to reveal the mechanism of the carcinogenesis of environmental and occupational hazards.
引用本文（GB/T 7714）
Ke-xia Yan, Bingci Liu, Ming Xu, 等. [Recombination and identification of sense and antisense cyclinD1 eukaryotic expression vectors].[J]. PubMed, 2003.
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