[Study on the scavenging action of polysaccharide of Usnea longissima to oxygen radical and its anti-lipi peroxidation effects].
摘要
OBJECTIVE: To study the scavenging action of polysaccharide of Usnea longissima Ach. (PUS) to oxygen free radical and its inhibiting action on lipidperoxidation. METHODS: The scavenging action to superoxide anion free radical (O2-.) was tested by xanthine-xanthine oxidase system. The scavenging action on hydroxyl free racical (OH.) and anti-lipidperoxidation effects were tested by Fenton reaction. RESULTS: The scaverging action was represented by the content scavenging 50% of all the free radical(IC50). The IC50 for O2-. and OH. were 0.45 mg/ml and 1.57 mg/ml respectively. The lipidperoxidation induced by OH. was weakly inhibited by PUS. CONCLUSION: PUS can apparently scavenge oxygen free radical. But it can only weakly inhibit the lipid peroxidation of hepatocyte homogenate of mice which resulted form hydroxyl free radical.