[Establishment of a method for detecting transforming growth factor beta 1 mRNA].
摘要
AIM: To establish a method to measure the TGF-beta 1 mRNA level for studying the mechanism of fibrogenesis caused by schistosomiasis japonica. METHODS: Reverse-transcription polymerase chain reaction and dot blot analysis were used. A plasmid of TGF-beta 1 was constructed for standardization, and beta-actin was used as control. Eight different concentrations of the plasmid and 11 double-tube of TGF-beta 1 mRNA in the peripheral blood mononuclear cell (PBMC) of different persons were measured. RESULTS: Quantitative results of 8 different dilutions of TGF-beta 1 plasmid had positive with the logarithm of the original concentration. The results of the 11 double-tube were 1.71 +/- 0.90 and 1.54 +/- 0.88. CONCLUSION: The duplicability and stability of the method showed it can be used to analyse the TGF-beta 1 mRNA level of the peripheral blood mononuclear cells.