[Proteomics of follicular fluid in mature human follicles and antral follicles: a comparative study with laser desorption/ionization-time of flight-mass spectrometry].
摘要
OBJECTIVE: To study the differentially expressed proteins between mature and antral follicles and identify the proteins crucial for follicle development and oocyte fertilization. METHODS: Mature follicular fluids were obtained from 48 women after oocyte collection during in vitro fertilization (IVF), and antral follicular fluids were collected from 21 women by follicular puncture. The proteins in the follicular fluids were detected by surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) and weak cation-exchange protein chip (WCX-2). The data were read with PBSII-C type protein chip reader and analyzed with Biomarker Wizard and Biomarker Patterns Software of Ciphergen Company. RESULTS: In comparison with those in the antral follicular fluid, the concentration of 4 proteins were significantly different in mature follicular fluid, including two up-regulated and two down-regulated proteins. CONCLUSION: Significant variation occurs in the proteomics of mature follicular fluid, and the differentially expressed proteins may have close relation to follicle development and oocyte fertilization.