[Protective immunity induced by recombinant signaling protein 14-3-3 vaccine of Schistosoma japonicum].
摘要
OBJECTIVE: To evaluate the immunoprotective effect of Schistosoma japonicum (Chinese strain) recombinant signaling protein 14-3-3 (rSj14-3-3), and to observe the synergism of rSj14-3-3 and rSjGST proteins as candidate vaccine and the effect of gamma delta-T cells activated by Mtb against Schistosoma japonicum. METHODS: BALB/c mice immunized with rSj14-3-3 and rSjGST purified through SDS-PAGE, electroelution and dialysis were challenged by cercaria infection. Six weeks after challenging infection, the mice were killed and the worm and egg reduction rates were calculated. RESULTS: Worm reduction rate was found to be 32.20% in rSj14-3-3 + Freund adjuvant group, 31.10% in rSj14-3-3 + rSjGST + Freund adjuvant group, 27.96% in rSj14-3-3 + Mtb group, 26.00% in rSj14-3-3 + rSjGST + Mtb group, and 27.10% in rSjGST + Mtb group, respectively, number of eggs in liver tissue was reduced by 50.40%, 53.30%, 51.10%, 58.60% and 51.30%, respectively. CONCLUSION: rSj14-3-3 could induce partial immunity against Schistosoma japonicum in BALB/c mice, and might serve as a candidate vaccine; gamma delta-T cell activated by Mtb played a role in anti-Schistosoma japonicum similar to the immune reactions induced by Freund adjuvant, but no synergistic effect combined with rSjGST was observed.