[Expression of P-glycoprotein and multidrug resistance-associated protein in peripheral blood mononuclear cells from multidrug resistant tuberculosis patients].
摘要
OBJECTIVE: To evaluate the expression of P-glycoprotein (P-pg) and multidrug resistance-associated protein (MRP) mRNA levels in peripheral blood mononuclear cells from multidrug resistant tuberculosis (MDR-TB) patients. METHODS: The subjects of this study included 3 groups: a non-TB control group, a TB control group and a MDR-TB group. The 31 subjects in the non-TB control group were staff from Wuhan Tuberculosis Prevention and Treatment Institute. The 55 cases in the TB control group were in-patients during September 2004 to December 2007 who were diagnosed as having pulmonary tuberculosis. The 89 cases in the MDR-TB group were in-patients during the same period, but who were diagnosed as having MDR-TB. Peripheral mononuclear cells were isolated and mRNA levels of P-pg and MRP were measured by real-time PCR. Comparisons of the data between different groups were performed using one-way ANOVA, and SNK q was used for comparison between 2 groups. RESULTS: There was no significant difference in the relative P-pg mRNA levels among the MDR-TB group (1.34 ± 0.32), the non-TB control group (1.05 ± 0.16) and the TB control group (1.12 ± 0.23), F = 0.536, P > 0.05. The relative MRP mRNA level (3.45 ± 0.43) was the highest in the MDR-TB group (3.45 ± 0.43), as compared to the TB control group (1.23 ± 0.34) and the non-TB control group (1.04 ± 0.12), F = 24.241, P < 0.05. CONCLUSION: Higher expression of MRP in peripheral mononuclear cells might be related to multidrug resistance in MDR-TB patients.