Immunogenicity of Yunnan-cobra Venom Factor in Non-human Primates
摘要
Cobra venom factor (CVF) depletes complement C3 and may therefore be\nimportant in preventing the complement-mediated damage and disease. In\ncomparison with various CVF reported previously, Yunnan-cobra venom\nfactor (Y-CVF) has higher anticomplement activity. The goal for this\nresearch is to investigate whether Y-CVF could induce the specific\nneutralized anti-Y-CVF antibody and xenoantibodies in non-human\nprimates. Thus two cynomolgus monkeys were intravenously injected with\nY-CVF (0.05 mg/kg) every two weeks for totally four times. The serum\nC3, CH50, anti-Y-CVF antibody and xenoantibody levels were measured at\ndifferent time points before and after Y-CVF injection. The results\nrevealed that, complement C3 was effectively depleted during the first\ntwo injections, and incompletely depleted during the third injection,\nbut almost not depleted during the last injection. The results of\nWestern blot and ELISA confirmed the production of anti-Y-CVF antibody,\nand its titer increased with the injection. Additionally no significant\nchanges of anti-porcine endothelia cell xenoantibodies were found\nmeasured by flow cytometry. We concluded that multiple injection of\nY-CVF stimulated anti-Y-CVF antibody production in monkeys, which\nresulted in the invalid of Y-CVF. The induction of anti-alpha -Gal\nxenoantibody by Y-CVF in primates was not observed.