Study of Periplaneta americana L.reversing multi-drug resistance of hepatocellular carcinoma
摘要
Objective To explore the effect of Periplaneta americana reversing multiple drug resistance(MDR) ofhepatocellular carcinoma in vitro.Methods Drug resistance of Hepatocellular carcinoma cell line HepG2/ADM was established by Adriamycin(ADM) increasing concentration gradient method; determined cell growth curve,doubling time change using Trypan blue staining method; detected sensitivity of 4 kinds of chemotherapeutic drugs of HepG2 and HepG2/ADM by methyl thiazolyl tetrazolium method; detected the non toxic dose of Periplaneta americana CⅡ-3 and the skim cream of HepG2/ADM by MTT method; detected the inhibition rate differences of CⅡ-3,skim cream,ADM,CⅡ-3 and ADM,skim cream and ADM ofhepatocellular carcinoma drug resistance cell line HepG2/ADM by MTT method.Results Drug-resistant cell line doubling time was extended obviously compared with parent cells,HepG2/ADM showed resistance to variety of chemotherapeutic drugs,ADM resistant index was especiallyhigh,that was 15.25 times.When the concentration of CⅡ-3 and skim cream were 28.76 and 45.08μg/mL respectively,the growth inhibition rate of HepG2 and HepG2/ADM two cell lines were 10%,no obvious toxic effect.The inhibition rate of the drug-resistant cell of 7.5,15,30μg/mL concentration CⅡ-3 joint with ADM concentration(1 /2 IC50) were(24.52 ± 2.05) %,(34.78 ± 3.08) %,(45.09 ± 3.56) % respectively; The inhibition rate of the drug-resistant cell of12.5,25,50μg/mL concentration skimmed cream joint with ADM concentration(1 /2 IC50) were(19.28 ±2.56) %,(35.08 ±2.13) %,(43.85 ±3.05) %respectively.Conclusion The cell line HepG2/ADM establishedhas basic multidrug-resistant biological characteristics.Periplaneta americana extract can inhibit the growth of HepG2/ADM,andhas good effect to reverse drug resistance at the same time.