[The biological effect of high glucose on human periodontal ligament fibroblast].
摘要
PURPOSE: The investigate the effect of high glucose on proliferation, total protein synthesis, alkaline phosphatase (ALP) activities, collagen-I(Col-I) and osteocalcin(OCN) secretion of human periodontal ligament fibroblast(hPDLF) in vitro and the regulative function of insulin in this process. METHODS: Glucose of different concentration (5.5,15,25,35and 45mmol/L) was used to culture hPDLF and insulin was used for each group as treatment control. 24 hrs after administration, cell proliferation was detected by cell counting kit (CCK-8), total protein synthesis, ALP activities, Col-I and OCN secretion were investigated. Data was analyzed with SPSS 13.0 soft ware package. RESULTS: Glucose of low concentration had no effect on biological function of hPDLF, but glucose of high concentration could inhibit proliferation, total protein synthesis, ALP activities, Col-I and OCN secretion of hPDLF significantly in a concentration-dependent manner. This effect could be blocked by insulin. CONCLUSIONS: High glucose could inhibit biological function of hPDLF and such effect could be blocked by insulin.