[Characteristics of staphylococcal enterotoxin B (SEB)-induced anergy in transplantation of allogeneic bone marrow cells].
摘要
AIM: To investigate the characteristics of the SEB-induced anergy in transplantation of allogeneic bone marrow cells. METHODS: C57BL/J (recipients) and BALB/c (donors) mice were used in this study. The recipients were irradiated with 6.0Gy (60)Co-gamma ray followed by transplantation of allogeneic bone marrow cells (6x10(7)). The recipients treated with (60)Co-gamma-ray were then divided into 3 groups; group 1 treated with (60)Co-gamma-ray (RI control group), and group 2 (transplantation control group) and group 3 (SEB experimental group) received saline or 60 mug SEB after the transplantation of bone marrow cells. T cell subtypes and expression of MHC molecules were detected by flow cytometry. Proliferation of lymphocytes in response to ConA and allogeneic antigen were examined by MLR. RESULTS: (1) The recipients treated with and without SEB survived for at least 180 days and became chimeric with white hair color (the donors' color). (2) SEB inhibited proliferation of lymphocytes in the recipients in response to ConA but not to allogeneic antigen as compared with that in the Group 1 and Group 2 mice. (3) The CD4(+) T but not CD8(+) T cells in the recipients treated with SEB were decreased from day 30 to day 60 after transplantation. (4) With the treatment of SEB, frequency of CD3(+)/NK1.1(+) NKT cells was increased to 5.71% on day 180. In the recipients, the expression of donor's MHC H-2K(d) were enhanced 80.95% and of expression self MHC H-2K(b) was 1.45% only. CONCLUSION: SEB can induce a stronger anergy than that induced by irradiation. SEB-induced anergy is related with the reduction of CD4(+) T cells and increase of CD3(+)/NK1.1(+) NKT cells at the early phase of transplantation. T cells showed specific down-regulation in this study.