Assignments of ~1H and ~(13)C NMR Signals of Mogroside IVa
摘要
Aim To investigate the structure of mogroside IVa isolated from traditional Chinese medicine fructus momordicae [fruits of Siraitia grosvenori (Swingle) C. Jeffery] and summarize the NMR characteristics of the structure. Methods Common extraction, separation and purification methods were used. Various NMR techniques including 1H NMR, 13 C NMR, DEPT, 1H 1H COSY, HSQC, HMBC, NOESY and molecular model simulated by computer were used to elucidate the structure. Results 1H and 13 C NMR signals of mogroside IVa were assigned, and spectroscopic basis was obtained for identification of such type of compounds. Conclusion 1D and 2D NMR techniques including 1H 1H COSY, HSQC, HMBC, NOESY spectra are powerful tools for structure analysis. The structure determined by NMR methods is identical with energy minimized conformation simulated by computer.