[On molecular identification and taxonomic status of Anopheles lesteri and Anopheles anthropophagus in China (Diptera: Culicidae)].
摘要
OBJECTIVE: To clarify the taxonomic status of Anopheles lesteri and An. anthropophagus in China. METHODS: Using molecular identification (PCR assay and rDNA-ITS2 sequencing) to examine the field anopheline mosquito specimens from Liaoning and Shandong. According to the ITS2 sequences, molecular phylogenetic tree was made. RESULTS: According to the molecular identification, An. lesteri and An. anthropophagus were distributed both in Liaoning Province and Shandong Province. The length and GC content of rDNA-ITS2 sequence were 451 bp, 46.2% in An. lesteri (n = 6), and 448 bp, 46.0% in An. anthropophagus (n = 10), respectively. The ITS2 sequences from presentation sites were same in An. lesteri, while the intraspecies difference in An. anthropophagus was 0.88%. The specific difference between An. lesteri and An. anthropophagus was 25.7%. By analyzing molecular phylogenetic tree, the relationship between An. lesteri and An. sinensis, An. anthropophagus and An. liangshanensis was found to be closer. CONCLUSION: According to the molecular identification, it was defined that An. lesteri and An. anthropophagus were sympatric independent species in China.