Spectrofluorimetric Determination of Tetracycline Using Eu 3+ Chelation and Cetyltrimethylammonium Bromide *
摘要
The fluorescence of tetracycline was greatly enhanced by chelation with Eu 3+ in Tris HCl buffer (pH8.5). The addition of cetyltrimethylammonium bromide, a cationic surfactant, further enhanced the fluorescence of tetracycline Eu 3+ chelates.This spectrofluorimetric method for the determination of tetracycline yielded a linear responeses in the range of 10 nmol/L 10 μmol/L. The detection limit was 5 nmol/L (or 2.22 ng/mL).The recovery of tetracycline spiked in serum at levels of l and 0.09 μg/mL was 97.9% and 97.8%, respectively. This method is fast, sensitive and suitable for the determination of tetracycline in biological specimens.
引用本文（GB/T 7714）
Huang Zu. Spectrofluorimetric Determination of Tetracycline Using Eu 3+ Chelation and Cetyltrimethylammonium Bromide *[J]. 未知来源, 1999.
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