[Experimental studies on the apoptosis of HL-60 cells induced by Brucea javanica oil emulsion].
摘要
OBJECTIVE: To investigate the inducing effect of Brucea javanica on the apoptpsis of HL-60 cells. METHOD: HL-60 cells were treated with Brucea javanica 1:100, 1:40, 1:20 (v/v) respectively for 6 h and DNA agarose gel electrophoresis, flow cytometry, fluorescence microscope, electron microscope were used to observe the apoptosis inducing effect of Brucea javanica. RESULT: DNA ladder was seen in the 1:40 group. The apoptosis cell percentages of 1:40 and 1:20 group were 86.8% and 97% respectively. Cells of 1:40 group showed obvious apoptosis character under fluorescence microscope. Cells were induced apoptosis in 1:20 and 1:40 Brucea javanica under electron microscope. CONCLUSION: 1:20 and 1:40 Brucea Javanica showed obvious apoptosis inducing effect of HL-60.