Expression pattern and function of SATB1 in the invasiveness of thyroid carcimoma
摘要
Objective To study the effect of SATB1 on invasiveness and metastasis of thyroid carcinoma cells,and to explore its molecular mechanisms to promote invasion and metastasis in thyroid carcinoma. Methods Construction of over expression plasmid for SATB1 gene,transfection of thyroid carcinoma SW-579 cells,transfection efficiency and epithelial cell marker protein of E-cadherin and Western blotting method for determination of qRT-PCR( E-cadherin) and mesenchymal cell marker vimentin( Vimentin) expression; qRT-PCR further detection of transcription factor Twist1 and Snail1,matrix metalloproteinases MMP2 and MMP9,as well as the the expression of inflammatory factors IL-6 and IL-8 changes; cell morphology was observed under an inverted microscope; cell wound healing assay and Transwell were used to detect the expression of SATB1 after thyroid cancer cell migration,invasion ability changes. Western blotting for detection of SATB1 enhanced the expression changes of Akt phosphorylation,PI3 K /Akt pathway inhibitor LY294002 cells was observed after overexpression of SATB1 in SW579 cell migration and invasion ability changes. Results The experimental results showed that cell migration,over expression of SATB1 after each perspective transfer cells( 196 ± 10),compared with the control group( 107 ±12),had a significantly increased( P 0. 01); invasion and experimental results showd that,after overexpression of SATB1 through the Matrigel cell number( 158 ± 12),compared with the control group( 86 ± 15),which significantly increased( P 0. 01). Western blotting found that after the transfection of SATB1 in total Akt protein,no change of circumstances,SW-579 cells,the phosphorylation of Akt( p-Akt) increased significantly. But suppressed by PI3 K /Akt signaling pathway inhibitor LY294002,observed cell migration and invasion ability decreased significantly. Conclusion SATB1 is associated with invasion and metastasis of thyroid carcinoma; its high expression is the promotion of thyroid ca