[Pharmacognostical studies on Baibu radix stemonae and allied drugs. IX. Determination and evaluation of total alkaloid content in the roots of Chinese Stemona spp].
摘要
An acid-dye colorimetric method was reported for the determination of total alkaloids in 53 samples of Baibu drugs from their growing destricts in 14 provinces and municipalities and its average recovery and its linear range were 96.1% (CV less than 4%) and 20-150 micrograms respectively. The relationship between the total alkaloid content, geographical origins and morphology were discussed. The results showed that: 1. the content of total alkaloids of stemona was 0.26-3.1%; 2. that of Stemona sessilifolia was 0.26-2.17% with the highest content of the sample from Nanyang country in Henan Province; 3. that of S. japonica was 0.83-1.43%; 4. that of S. tuberosa was 0.53-3.1% with the highest content of sample from Hengyang in Hunan Province; 5. that of S. parviflora was 0.22-0.74% with the highest content of sample from Qongzhong in Hainan Province; and 6. that of more yellow, solider and stronger samples was higher than that of any other samples. However, that of all bigger samples in shape was not higher than that of smaller ones.