[Experimental study on cell adhesion characteristic between porous biphasic calcium phosphate nanocomposite and bone marrow mesenchymal stem cells in vitro].
摘要
OBJECTIVE: To study the adhesion characteristic in vitro between porous biphasic calcium phosphate (BCP) nanocomposite and bone marrow mesenchymal stem cells (MSCs) that have been induced and proliferated. METHODS: MSCs obtained from SD rat bone marrow were in vitro induced and proliferated. After their osteoblastic phenotype were demonstrated, MSCs were seeded onto prepared porous BCP nanocomposite (experiment group)and common porous hydroxyapatite (control group). Their adhesion situation was analyzed by scanning electron microscope. The initial optimal cell seeding density was investigated between new pattern porous BCP nanocomposite and MSCs by MTT automated colorimetric microassay method. RESULTS: The differentiation of MSCs to osteoblastic phenotype were demonstrated by the positive staining of mineralized node, alkaline phosphatase (ALP) and collagen type I, the most appropriate seeding density between them was 2 X 10(6)/ml. The maximal number which MSCs could adhere to porous BCP nanocomposite was 1.28 X 10(7)/cm3. CONCLUSION: MSCs can differentiate to osteoblastic phenotype. The MSCs were well adhered to porous BCP nanocomposite.