RADIOIMMUNOASSAY ON SERUM GONADOTROPIN OF CARP (Cyprinus carpio L.)
摘要
The mixed pituitary glands of Cyprinus carpio, Carassius auratus, Hypophthalmichthys molitrix and Aristichthys nobilis were extracted with ethanol. Then the crude gonadotropin was fractionated by chromatography on DEAE-cellulose and gel filtration. Biological activity as assayed by ovulation test of the loach (Misgurnus anguillicaudatus) in vivo was recognized only in the second fraction (Sephadex G-100). Effective dose of induced ovulation was 1μg/g body weight. The gonadotropin (SG-Ⅱ-2) exhibited a single band on polyacrylamide gel electrophoresis. The determinable range of this gonadotropin radioimmunoassay was 0.1～16ng.Serum gonadotropin levels of carp (Cyprinus carpio) have been determined by means of radioimmunoassay through the whole reproductive process. Particularly the female exhibited a significant and higher peak during the sexual behaviour and spawning. After the spawning, the serum gonadotropin levels have a quick drop down. This investigation provided some new data for reproductive physiology of fish.