[Reversion of the malignant phenotype of gastric cancer by c-erbB-2 specific ribozyme].
摘要
OBJECTIVE: To probe the effect of c-erbB-2 specific ribozyme to the malignant phenotype of gastric cancer. METHODS: The eucaryotic expression vector of c-erbB-2 specific ribozyme RZ1 was designed by computer and named pDOR-RZ1. The transfection of the gastric cancer cell line SGC-7901 was mediated by Lipofect AMINE. Flow cytometry was used to analyse the expression of the c-erbB-2 product P185. In vivo study of tumorogenecity of the transfected cells was performed in nude mice. RESULTS: pDOR-RZ1 was successfully transfected into the gastric cancer cell line SGC-7901 and then the single clones were selected by G418 and named SGC/RZ1. Flow cytometry analysis showed that the expression of P185 was suppressed by 62.7%. The growth rate of SGC/RZ1 was inhibited by 55%. The tumor-forming time in SGC/RZ1 in nude mice was delayed remarkably and the tumor size was also much smaller than that in the control group, indicating the inhibition of the tumorogenecity of SGC/RZ1 in nude mice. CONCLUSION: c-erbB-2 specific ribozyme is very efficient in reversing the malignant phenotype of gastric cancer. This might provide a new approach for gene therapy of gastric cancer.