[Expression of VEGF and NGF in gliomas of human].
摘要
OBJECTIVE: To examine the expression of vascular endothelial cell growth factor (VEGF) and nerve growth factor (NGF) in gliomas of human, and investigate the relationship between VEGF, NGF and pathologic grading as well as tumorigenesis of gliomas. METHODS: Immunohistochemistry (SP) and Western blot were applied to evaluate the expression of VEGF and NGF in 5 cases of normal controls and 20 cases of human gliomas. RESULTS: VEGF and NGF were expressed in both normal brain tissues and gliomas. VEGF was observed mainly in the cytoplasm of gliomas, while NGF were occurred in both cytoplasm and nucleus of gliomas. Western blot analysis demonstrated that the expression level of VEGF and NGF was significantly higher than that in normal controls (P< 0.05), and a significant difference in vary pathologic grading gliomas was also observed. CONCLUSION: VEGF and NGF were expressed in both the normal brain tissues and gliomas. The level of VEGF and NGF in gliomas was upregulated than that of the normal controls. The results indicate VEGF and NGF may play an important role in the tumorigenesis of gliomas, and relate to the extent of gliomas malignant.