EFFECT OF SOMATOSTATIN ON THE CELL CYCLE OF HUMAN GALLBLADDER CANCER CELL
摘要
Objective To explore the effect of somatostatin on the cell cycle of human gallbladder cancercell. Methods Growth curve of gallbladder cancer cell was measured after somatostatin treated on gradientconcentration. Simultaneously, the change of gallbladder cancer cell cycle was detected using flow cytometry.Results Concentration-dependent cell growth inhibition caused by somatostatin was detected in gallbladder cancercell(P 0. 05). Cell growth was arrested in S phase since 12h after somatostatin treated, which reached its peak at24h, then fell down. The changes in apoptosis index of gallbladder cancer cell caused by somatostatin correlatedwith that's in cell cycle. Conclusion Somatostatin could inhibit the cell growth of human gallbladder cancer cellin vitro on higher concentration. It might result from inducing growth arrest in S phase in early stage and inducingapoptosis in the late stage.