[Identification of metastasis-associated proteins of ovarian cancer by proteomics].
摘要
OBJECTIVE: To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities. METHODS: The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach. RESULTS: Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system. CONCLUSION: The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.