[Cloning the 5 ' end fragment of ST13 cDNA by nested PCR].
摘要
OBJECTIVE: To clone and sequence the 5' -end fragment of ST13 cDNA. METHODS: The 5' -end fragment of the ST13cDNA was amplified directly from cDNA library by nested PCR method, and cloned into pGEM-T. easy vector, then the sequencing of the inserted PCR product was performed. RESULTS: After primary and secondary PCR, two PCR products obtained with the size of about 550bp and 480bp, respectively, were both proved to be the 5' -end of ST13 cDNA by sequencing. CONCLUSION: the method presented is very simple and effective for cloning and sequencing the 5'-end of a target gene.
引用本文（GB/T 7714）
Lili Geng, Cai X, Jun Cao, 等. [Cloning the 5 ' end fragment of ST13 cDNA by nested PCR].[J]. PubMed, 1999.
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