[Tissue culture and plant regeneration of Bolbostemma paniculatum].
摘要
The tissue culture and plant regeneration of Bolbostemma paniculatum were studied and a large number of regenerated plantlets were obtained. The optimal compounding of medium that induced calli from stems and leaves were the MS medium supplemented with 2,4-D 2.0 mg/L, NAA 0.5 mg/L and BA 1.0 mg/L and the MS medium with 2,4-D 0.5 mg/L and NAA 2.0 mg/L, respectively. Numerous shoots could formed directly when leaf explants were cultured on the MS medium with BA 3.0 mg/L and NAA 1.0 mg/L and calli were cultured on the MS medium with BA 2.0 mg/L and IAA 1.0 mg/L. The MS medium supplemented with BA 2.0 mg/L, IAA 0.1 mg/L was optimal for shoots growth. For the root growth was 1/2 MS medium with 1.0 mg/L IBA. At pH 6.0 and 0.8% - 1.0% agar was optimal for callus and shoot formation, while pH 5.8 and 0.6% - 0.7% agar was optimal for root formation. The tube seedling can be successfully transplanted.