PREPARATION AND CRYSTALLIZATION OF DES (B-CHAIN C-TERMINAL) HEPTAPEPTIDE INSULIN
摘要
An insulin analogue, des(B-chain C-terminal) heptapeptide insulin (DHPI), has been prepared from des(B-chain C-terminal) pentapeptide insulin (DPI) by the limited hydrolysis with carboxypeptidase A at pH 7.4, 0℃, for 10hr. The desired product, collected from the hydrolysate through Sephadex G-50 column chromatography, has been identified by chemical analysis of the C-terminal amino acid, C-terminal sequence and amino acid composition, and polyacrylamide gel plate electrophoresis. DHPI can crystallize in the form of platelets in a 0.05M citrate buffer, pH 5.9-6.1 containing 33% acetone and a trace of ZnAc_2. Pure DHPI is inactive by the mouse convulsion test.
引用本文（GB/T 7714）
Lu Zhang. PREPARATION AND CRYSTALLIZATION OF DES (B-CHAIN C-TERMINAL) HEPTAPEPTIDE INSULIN[J]. 未知来源, 1980.
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