[Effect of effective fractions and its compatibilities and proportions of xie-xin decoction on nitric oxide production in peritonea macrophages from rat].
摘要
OBJECTIVE: To observe the effect of effective fractions (Conjunct anthraquinone, free anthraquinone and total flavonoids) and its compatibilities and proportions of Xie-Xin decoction on NO production in peritonea macrophaes from rat. METHODS: Growth activity of macrophages cultured with different levels of active components were detected by MTT. NO concentrations in peritoneal macrophages induced by LPS were detected by Griess method. RESULTS: The NO production from macrophages induced by LPS was inhibited obviously by active components at the levels of 0.01-0.1 mg/ml. The best time of administration was 1 h induced by LPS. The inhibition of best proportions of compatibilities of Conjunct anthraquinone and total flavonoids, free anthraquinone and total flavonoids were stronger than effective fractions solo. CONCLUSION: The NO production is inhibited obviously by these effective fractions, especially by conjunct anthraquinone. Effective fractions can inhibit the function of activated macrophages.