基于标准光盘/光驱的数字化分子检测技术研究进展
摘要
Traditional medical diagnosis is typically time-consuming and labor-intensive, which is currently limited to well-equipped laboratories and hospitals where the required special instrumentation and highly trained personnel are available. Since patients cannot obtain their test results on time, the consequence of delayed treatment can be disastrous. Therefore, affordable and simple point-of-care (POC) biosensor devices are needed to perform diagnoses in the clinic or even at home. Compact disc (CD) technology is promising for the development of inexpensive materials and tools for POC molecular screening; while the discs may offer versatile biochip fabrication materials, a standard computer optical drive can be adapted as a precise signal readout device. In this mini-review, we briefly describe our recent progress in the development of computer-readable disc-based bioassays, including the UV/ozone activation of the polycarbonate surface, the preparation of DNA hybridization/antibody-antigen binding bioassays, and the establishment of a digital protocol for signal reading.