氯化镧抑制前列腺癌细胞DU145 的生长和迁移
摘要
Lanthanides compounds have been reported to perform anticancer activity in certain cancer cells, but there have been few reports looking specifically at the effects of these compounds on prostate cancer. In this study, we evaluated the effects of lanthanum chloride (LaCl<sub>3</sub>) on the proliferation, adhesion and migration ability of androgen-independent prostate cancer DU145 cells. The results of MTT assay showed that LaCl3 treatment inhibited the proliferation of DU-145 cells in a dose-dependent manner. Using colony forming assay, LaCl<sub>3</sub> treatment led to reduced colony forming ability of DU145 cells. Further observations demonstrated that the presence of LaCl<sub>3</sub> decreased adhesive capacity, cell-surface area and migration in DU145 cells. In addition, LaCl<sub>3</sub> treatment inhibited phosphorylation of extracellular signal-regulated kinase (ERK) and P38 MAPK kinase of DU145 cells. Moreover, pretreatment of the cells with pertussis toxin (PTx), a Gi protein inhibitor, abolished LaCl<sub>3</sub>-induced ERK and P38 phosphorylation, as well as colony formation and cell-surface area. In conclusion, the findings suggest that LaCl<sub>3</sub> inhibits in vitro DU145 cells growth and migration, and the effect is partly linked to Gi protein signaling pathway.