功能化氧化石墨烯的靶向肿瘤成像与光热治疗
摘要
Integrin α<sub>v</sub>β<sub>3</sub> is a heaterodimeric transmembrane receptor, overexpressed in some types of tumor cells such as glioblastoma U87-MG cells. Integrin α<sub>v</sub>β<sub>3</sub>, as a tumor biomark, is widely used in cancer diagnosis and therapy. In this work we propose an active targeting nanocarrier system based on nano-graphene oxide (NGO-mAb-FITC) for imaging and photothermal therapy (PTT), in which glioblastoma U87-MG cells are used as the model of tumor therapy. The nanoprobes efficiently target U87-MG cells (integrin α<sub>v</sub>β<sub>3</sub>-positive), with minimal influence on MCF-7 cells (integrin α<sub>v</sub>β<sub>3</sub>-negative). These NGO-mAb-FITC nanoprobes are then internalized into the α<sub>v</sub>β<sub>3</sub>-positive tumor cells. Under 808 nm light irradiation, cells can be ablated by hyperthermia-induced apoptosis. The results indicate that the graphene oxide, a potential photothermal transduction nanoprobe, may potentially serve as a novel probe for cancer targeted imaging and therapy.