梧州繁殖中心圈养黑叶猴遗传多样性分析和野外放归种源选择
摘要
Mitochondrial DNA (mtDNA) control region and microsatellite DNA sequences were used to analyze genetic diversity, origins, and relationships of captive langur ( Trachypithecus francoisi ) individuals. Sequencing of the 355-bp mtDNA control region for 52 individuals uncovered 35 variable nucleotide sites, including 3 transitions (ts), 29 transversions (tv), and 3 insertion/deletions, with 13 defined haplotypes. A haplotype diversity of 0.627 and a nucleotide diversity of 0.027 were calculated. Eleven microsatellite loci showing good amplification were also assayed in the 52 individuals. A total of 47 alleles were detected, with an average of 4.18 per locus. Mean polymorphic information content ( PIC ) was 0.566. Expected heterozygosity ( He ) and observed heterozygosity ( Ho ) were 0.559 and 0.551. Compared with other endangered primates, we found that the genetic diversity of the captive T. francoisi population was not low. Based on phylogenetic analysis of haplotypes of wild and captive T. francoisi individuals, we inferred that the captive T. francoisi individuals came from Guangxi, near the Vietnamese border. Using genetic distance relationships, we selected three male and seven female captive langurs to establish three family units for reintroduction.