Protective Effects of Tea Polyphenols on Cerebral Nerve Cell Apoptosis Induced by D-galactose and Beta-amyloid Peptide 25-35
摘要
BACKGROUND: Some researches demonstrate that tea polyphenols (TP) has protective effects on neurotoxicity of hippocampal nerve cells induced by β-amyloid peptide (Aβ), 6-hydroxydopamine (6-OHDA) and oxidative substances. In addition, clinical preliminary examination indicates that TP plays a certain preventive and therapeutic effects on the reduction of recognition function in high-risk population with Alzheimer disease (AD); however, its target and mechanism are still hot topics. OBJECTIVE: To observe the interfering effects of TP on cerebral nerve cell apoptosis induced by D-galactose and Aβ(subscript 25-25) in mice. DESIGN: Randomized controlled animal study. SETTING: Department of Pharmacology, Pharmacological College of Jinan University. MATERIALS: The experiment was carried out in the Experimental Center of Jinan University from September 2004 to January 2005. A total of 90 healthy Kumning mice, aged 2 months, each gender in half, weighing 26-28 g, were provided by Guangdong Provincial Medical Laboratory Animal Center. Tea polyphenols was provided by Zhejiang Oriental Tea Science and Technology Corporation (batch number: 20040203); D-galactose by Shanghai Number 2 Reagent Plant (batch number: 20030708); Aβ(subscript 25-35) by Sigma (batch number: 13/0112004); vitamin E (Vit-E) by Shanghai Xinyi Pharmaceutical Factory (batch number: 20030708). METHODS: Experimental interference: Mice based on body mass were randomly divided into 6 groups: sham operation group (n=17), model group (n=16), vitamin E group (n=16), low-dose (n=13), moderate-dose (n=14) and high-dose (n=14) tea polyphenols groups. In above-mentioned animals, except those in the sham operation group, all were given 120mg/(kg•d) D-galactose for 12 consecutive weeks, and Aβ(subscript 25-35) (4nmol) was slowly injected into the lateral cerebral ventricle. In sham operation group, the same volume of artificial cerebral spinal fluid (CSF) was internally injected into lateral ventricle. Drug treatment