丹蛭降糖胶囊对糖尿病大鼠肾组织NF-κB表达的影响*
摘要
Objective: To investigate the expression level of NF- κ B in renal tissue of diabetic rodent model and the regulating action of Danzhi Jiangtang Capsule (DJC) . Methods : 55 male SD rats were randomly divided into 2 groups, normal control group and experimental group. Normal control group was fed with basal diet, the experimental group were fed with high calorie diet. A single intraperitoneal injection of streptozotocin (STZ) of 35mg/kg to establish the animal model of diabetes mellitus, blood glucose was measured after 120h,when the diabetic rodent model was built, the experimental group were randomly divided into 4 groups, high does group of DJC, low does group of DJC , pioglitazone group and model group. In addition to the model group and control group, each group was kept with continuous intragastric administration of drugs for 8 weeks, high does group of DJC, low does group of DJC were treated with 1.08g/ (kg • d), 0.54 g/ (kg • d) by gavage, pioglitazone group was treated with 10mg/ (kg • d) by gavage, the blank group and the model group were treated with physiological saline 5ml/ (kg • d) by gavage. Then determined NF-κB by immunohistochemical method and detected blood glucose of rats . Results: The expression of NF-κB in model group was strongly and significantly increased compared with the normal control group （ P ＜0.05 or P ＜0.01）; after being given Danzhi Jiangtang Capsule for 8 weeks , high expression of NF-κB in high DJC group and low DJC group were significantly decreased ( P ＜0.05), much more than pioglitazone group . When the diabetic rodent model was built, the blood glucose of model group was increased significantly compared with the control group ( P ＜0.01).After the experimental group been given Danzhi Jiangtang Capsule for 2 weeks , the level of glucose was decreased significantly compared with model group ( P ＜0.01) , the blood glucose index declined steadily after 4~8 weeks（ P ＜0.01）. Conclusion : Danzhi Jiangtang Capsule can reduce blood su