A Rapid Method to Establish Suspension Cultures of Paulownia Species
摘要
Cell suspensions were obtained within one month by culturing seedling shoots or plantlet leaves of Paulownia fortunei, P. kawakamii, P. tomentosa and P. taiwaniana in liquid media. MS medium supplemented with 1 mg/L2,4-D and 0.1 mg/Lkinetin was used. Cultures were kept in the dark on a gyratory shaker at 100 rpm. Cells with densities of 4.5 10^5 cells/mL were released directly from 200-mg explants cultured within 1-2 wk. Most cells (70-95%) were viable. Cultures of P. tomentosa and P. taiwaniana were maintained for more than one year by regularly subculturing, while P. fortunei and P.kawakamii failed after successive culturing. The same medium was used for the subculture of P. tomentosa cells, but for the subculture of P. taiwaniana, are duction in both 2,4-D and kinetin concentrations coupled with the addition of NAA and BA was necessary . This method is effective and timesaving compared to the generally accepted method using callus tissue. The production of friable calluses on agar suitable for suspension culture was eliminated.