Complexing cis-diaminedichloroplatinum-loaded Magnetic nano-medicine for Treating Nasopharyngeal Carcinoma in vitro
摘要
BACKGROUND: Anticancer drugs-loaded magnetic nanoparticles, as a novel targeting drug delivery system, are characterized by high drug loading dose, targeting location transport, heat effect of magnetic grains, and biological degradation. Thus, this system brings new hopes for chemical therapy with high efficiency and low toxic and side effects. OBJECTIVE: To observe in vitro toxic effects of complexing cis-diaminedichloroplatinum (CDDP)-loaded magnetic nanoparticles on human nasopharyngeal carcinoma (NPC) CNE2 cells. DESIGN, TIME AND SETTING: The in vitro controlled study was performed at the Laboratory of Pharmacology, Northern Region, Sun Vat-sen University in March 2005. MATERIALS: CDDP was provided by Shandong Qilu Pharmaceutical factory. CDDP-loaded magnetic nanoparticles (CDDP-SAMNP), 43-52 nm in particle diameter. Utilization rate of CDDP was about 65%. NPC CNE2 cell line was supplied by the Laboratory of Cell Pathology, Cancer Hospital, Sun Vat-sen University. METHODS: This study contained medication and control groups. The medication group was assigned to CDDP and CDDP-SAMNP groups. CDDP and CDDP-SAMNP were diluted by RPMI-1640 medium. Drug concentration was in accordance with CDDP content. The control group was divided into RPMI-1640 medium and SAMNP groups (adding ferroso-ferric oxide, magnetic nucleus concentration was 7 g/L, diluted by the medium). MAIN OUTCOME MEASURES: MTT assay was used to observe kill and wound rate of 1.89-11.34 mg/L CDDP and corresponding dose of CDDP-SAMNP on NPC CNE2 cells following 24 and 48 hours. Uptake of CDDP-SAMNP by CNE2 cells was investigated under a transmission electron microscope. RESULTS: SAMNP as the medium group had no effect on killing or wounding CNE2 cells (P＞0.05). With the increment of CDDP and CDDP-SAMNP dose, the kill and wound rate presented an obvious dose-effect relationship. At the same dose, the same medicine showed an increasing kill and wound rate with the extension of reaction time, presenting