Genotyping of Canine Parvovirus Type 2 VP2 Gene in Southern Taiwan in 2011
摘要
Canine parvovirus type 2 (CPV-2) is a non-enveloped single-stranded DNA virus that was first recognized in 1978. Since then it has undergone evolution still generating new genetic variants. CPV-2 infection is the most important infectious viral enteritis to dogs. The aim of this study was to genotype CPV-2 in southern Taiwan. Previous study in central Taiwan demonstrated ”CPV-2b” as major canine parvovirus strains. This study demonstrated that, by PCR-based genotyping alone, could not discriminate ”CPV-2a” from ”CPV-2b” strains, because the ”new CPV-2a” strain would be assigned as ”CPV-2b” by PCR-based genotyping assay. This study showed that 35 out of 54 specimens (65%) were ”new CPV-2a” as the major CPV-2 strain in southern Taiwan. Besides, the VP2 gene of Chiayi isolates from dogs revealed an amino acid substitution at Ile-324 for Tyr-324 in other CPV strains. Molecular characterization of CPV-2 typing may provide CPV-2 epidemiological surveillance, even to improve the disease control in Taiwan.