天麻藥材中指標成分Parishin, Parishin B及C之高效液相層析定量法之探討
摘要
A rapid method by high performance liquid chromatography was developed for the simultaneous determination of three marker substances, parishin, parishins B and C, in Gastrodiae Rhizoma. This determination was carried out by using a gradient elution with methanol and 0.1% phosphoric acid on a Cosmosil 5C18-AR column. The linear calibration range of parishin, parishins B and C were 80~l200μg/ml (r=0.9998), 120~760μg/ml (r=0.9831) and 40~320μg/ml (r=0.9995), respectively. The recoveries of the markers were parishin: 102.6±3.3%, parishin B: 102.8±2.7% and parishin C: 102.6±2.9%. The relative standard deviations of the three marker substances for intraday and interday analyses were 0.73~2.11% and 0.67~3.56%, respectively. The contents of the markers mentioned above in 23 crude drugs of Gastrodiae Rhizoma have been determined and the results were obtained as follows: parishin 0.37~1.50%, parishin B 0.12~0.65% and parishin C 0.08~0.27%. Therefore, HPLC is a useful technique to determine these selected markers.