ROLE OF OUTER MEMBRANE PROTEINS IN IMIPENEM DIFFUSION IN PSEUDOMONAS AERUGINOSA
摘要
The present study identified the properties of porins in the outer membrane in Pseudomomis arruginosa, and showed the role of outer membrane in determining [mlpenem diffusion in Pseudomonas arrgginosa. The molecular weight of the major outer membrane protein was analyzed by SDS-PAGE. The purification of the porins in Pseudomonas aeruginasa was achieved by DEAE ion - exchange HPLC. The purified outer membrane proteins were reconstituted with phosphatidylcboline and dicetylphosplmte into membrane vesicles, and were tested by the liposomes swelling method for the diffusion of imipermm. The permeability assay showed that OpN2 (70 kD), OprD2 (46kD), and OprE (4.3 kD) were the channel- forming proteins.But only OgrD2 was thought to be the likely route of imipenem diffusion.