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Reproductive characteristics of transgenic (TG) chickens carrying an exogenous gene

FumioEbara

1999Acta Scientiarum Naturalium Universitatis SunyatseniBiochemistry, Genetics and Molecular Biology被引 1

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摘要

An exogenous gene (lacZ/MiwZ) introduced into the germinal crescent region (GCR) of avian embryos was con-finned to be successfully transferred to the gonads via the primordial germ ceils (PGCs). Following hatching, the chinkswere raised until the stage of sexual maturation. The incorporation of MiwZ DNA was detected in male and female trans-genic chickens, respectively. The normal male and female transgenic birds were subjected to artificial insemfution according to routine methods. Fertilized eggs obtained from female transgenic chickens were incubated for 72 h and the em-bryos removed from the yolk were examined by X-gal staining to detect the introduction of MiwZ in the offspring. As a result,

引用本文(GB/T 7714)

FumioEbara. Reproductive characteristics of transgenic (TG) chickens carrying an exogenous gene[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 1999.

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