[Screening and functional properties of cholesterol-degrading lactic acid bacteria from Jiangshui].
摘要
OBJECTIVE: We intended to obtain and characterize lactic acid bacteria with high capacity of cholesterol-degrading. METHODS: We chose Jiangshui as the experimental material, screened lactic acid bacteria by the culture medium with high cholesterol, and studied other features of lactic acid bacteria like salt-tolerant, acid resistance, then identified the species of lactic acid bacteria by combining physiological and biochemical methods and 16S rDNA sequence. RESULTS: All lactic acid bacteria isolated had the capacity of cholesterol-degrading to some extent. There were 4 strains had high cholesterol-degrading rate (> 75%). Four strains were Lactococcus lactis subsp. lactis, two were Brevibacterium casei, and one was Lactococcus raffinolactis. CONCLUSION: Cholesterol-degrading lactic acid bacteria were screened from Jiangshui, with application potential for cholesterol degradation.