Dengue Virus Type 2 Unresponsive to the Current PCR Primer ; Construction of a New PCR Primer to Detect all Strains of Dengue Virus Type 2
摘要
We found that one strain of dengue virus (Trinidad 1751; TR) did not respond to the PCR primer for Jamaica/83. We investigated such property with other 10 strains of dengue virus type 2 and found 2 more unresponsive strains. All 3 strains were isolated from the central America. To detect the envelope gene of those 3 strains by PCR, we synthesized primers based on TR strain as the reference sequence. Using these primers, we could detect the 3 strains by PCR at the usual annealing temperature. We recommed the new primer for diagnosis of DEN 2.
引用本文（GB/T 7714）
Yosuke Kameoka, Noriko Hirota, Masami Kobayashi, 等. Dengue Virus Type 2 Unresponsive to the Current PCR Primer ; Construction of a New PCR Primer to Detect all Strains of Dengue Virus Type 2[J]. Nagasaki University's Academic Output SITE (Nagasaki University), 1995.
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