Synthesis and Characterization of a Sulfhydryl-Reactive Rhenium Metal−Ligand Complex
摘要
We describe the synthesis and spectral characterization of a rhenium metal-ligand complex. This complex reacts with sulfhydryl groups via an iodoacetamide side chain on the phenanthroline ligand and displays a high limiting anisotropy near 0.35 when excited at 442 nm. When covalently linked to human serum albumin, this complex displayed a mean decay time of about 1 micros. This decay time is appropriate for measuring rotational correlation times on the microsecond time scale as may occur for large macromolecular complexes.
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Jonathan D. Dattelbaum, Omoefe O. Abugo, Joseph R. Lakowicz. Synthesis and Characterization of a Sulfhydryl-Reactive Rhenium Metal−Ligand Complex[J]. Bioconjugate Chemistry, 2000.
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