Role of NF-kappaB in Liver Ischemia Reperfusion Injury of Rats
摘要
Summary: To determine the role of NF-kappaB in ischemia reperfusion (I/R) injury of the rat liver,rats underwent partial hepatic ischemia and reperfusion. The left and median lobes of the liver weresubjected to ischemia for 90 min followed by reperfusion for defined times. NF-kappaB activity wasanalyzed by electrophoretic mobility shift assay (EMSA). Semiquantitative reverse-transcriptasepolymerase chain reaction was used to analyze TNF-α and ICAM-1 mRNA levels. Results showedduring liver I/R injury, NF-kappaB activation was induced in a time dependent manner. NF-kappaBwas activated within 1 h and 2 h after the initiation of reperfusion and decreased after 4 h. MessengerRNA expression of TNF-α and ICAM-1 were increased after the reperfusion of 2 h. It was concludedthat during hepatic I/R injury, NF-kappaB was activated and could bind to special sequence in thepromoters of budget genes, which can up-regulate the expression of TNF-α and ICAM-1 mRNA toresult in ishemia reperfusion injury of the rat liver.