首页 / 资料库 / 文献详情

PURIFICATION AND SOME PROPERTIES OF CELLULASE FROM ODONTOTERMES FORMOSANUS （ISOPTERA： TERMITIDAE）

Tian-ciYang、Jian-chuMo、Jia-anCheng

2004Acta Scientiarum Naturalium Universitatis SunyatseniBiochemistry, Genetics and Molecular Biology被引 6

摘要

The purification of the cellulase from Odontotermes forrnosanus workers was achieved by using anion-exchange column of UNOsphere Q, BioLogic DuoFlow chromatography system. The purified cellulase was identified as an endoglucanase and some of its properties were investigated. The EGase activity was 807.5-fold as high as the initial enzyme activity using CMC as substrate and 14.4-fold using salicin as substrate. The enzyme preparations were homogeneous as judged by SDS-PAGE electrophoresis, molecular weight of which was 80 kDa and confirmed by 2-DE zymogram analysis. The enzyme was isoelectric at pH 6.4, which was active on CMC substrate.

引用本文（GB/T 7714）