Expression of HO-1 in Chronic Renal Insufficiency Rat Kidney and Implication
摘要
The expression, activity and clinical implication of heme oxygenase-1 (HO-1) in thechronic renal insufficiency (CRI) rat kidney and its mechanism were investigated. The 5/6 nephrec-tomized rats were assigned to sham operation group, CRI group and Hemin group. At the 8th weekafter second operation, blood pressure, urinary protein, serum creatinine(Scr) and BUN weremeasured. Renal pathologic changes were observed. The activity of HO and contents of erythropoi-etin (EPO) in serum and renal tissue were determined. Immunohistochemistry was used to detectthe expression and distribution of HO-1 in the CRI rat kidney. As compared with CRI group, theurinary protein, blood pressure, Scr and BUN in Hemin group were reduced significantly (P＜0. 05). The glomerular mesangial proliferation, inflammatory cellular infiltration of renal interstiti-um and interstitial fibrosis were ameliorated significantly. Immunohistochemistry and measurementof HO-1 activity revealed that the expression and activity of HO-1 was decreased in renal tissuesand increased in serum in CRI group as compared with normal rats. HO-1 distributed mainly in tu-bular epithelial cells. The EPO contents in Hemin group were significantly higher than in CRIgroup. Through up-regulating the EPO level in serum and renal tissues, HO-1 retards the progres-sion of CRI.